|
KMID : 0381920110410020123
|
|
Korean Journal of Microscopy
2011 Volume.41 No. 2 p.123 ~ p.128
|
|
|
Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization
|
|
Kim Seong-Hoon
Lee Chi-Hoon
Ju Hea-Sung
Kim Hyung-Bae
Lee Young-Don
|
|
|
Abstract
|
|
|
|
The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles (6.6±0.41 μm) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 μm, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructura characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.
|
|
|
KEYWORD
|
|
|
Blacktip grouper, Epinephelus fasciatus, Micropyle, SEM
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|